ILC2 Roles In Pulmonary Disease
Scope
This topic page describes how ILC2s are represented in the current ILC_in_lung wiki as disease-relevant cells in lung and airway contexts. It focuses on asthma/allergic airway inflammation, respiratory viral infection, post-viral repair, airway hyperreactivity, macrophage niche effects, and plastic or non-type-2 ILC2-like states.
This page expands the disease branch of ILC2. Use the entity page for the canonical cell-level model, then use this topic when the question is specifically about disease context and pathology.
Evidence tags
#cell/ILC2 #tissue/lung #outcome/airway_hyperresponsiveness #outcome/infection #outcome/repair #outcome/inflammation #axis/ILC_lung_infection #axis/ILC_airway_inflammation #axis/ILC_plasticity
Confidence snapshot
- High confidence: the local source set supports ILC2s as major contributors to type 2 airway inflammation, allergic asthma-like responses, and airway hyperresponsiveness.
- High confidence: the source set also supports reparative or tissue-protective ILC2 roles after respiratory viral injury.
- Medium confidence: ILC2 disease function is shaped by memory-like behavior, metabolic state, neuroimmune inputs, epithelial alarmins, and macrophage/niche interactions.
- Medium confidence: ILC2s can deviate from canonical type 2 output toward IL-17-producing or ILC3-like states in selected inflammatory contexts.
- Low confidence: the exact equivalence between mouse lung ILC2 states and human asthma or nasal-polyp ILC2 states remains unresolved in this wiki.
Established observations
Asthma and allergic airway inflammation
- In the local source set, asthma is the dominant disease setting for pathogenic ILC2 activity.
- ILC2s are linked to rapid type 2 cytokine output, especially IL-5 and IL-13, and to airway hyperresponsiveness and mucus-related pathology in multiple asthma/allergic airway sources, including Decoding innate lymphoid cells and innate-like lymphocytes in asthma pathways to mechanisms and therapies, Innate lymphoid cells and asthma, Innate lymphoid cells in asthma pathophysiological insights from murine models to human asthma phenotypes, and the review-level asthma regulation map Group 2 Innate Lymphoid Cells: Team Players in Regulating Asthma.
- Allergen-Experienced Group 2 Innate Lymphoid Cells Acquire Memory-like Properties and Enhance Allergic Lung Inflammation supports a disease-amplifying memory-like ILC2 branch in allergic lung inflammation.
- Innate lymphoid cells contribute to allergic airway disease exacerbation by obesity supports obesity as a mouse-model context in which allergic airway disease is exacerbated and both ILC2 and ILC3 responses can be altered; it should not be generalized to all human obesity-asthma phenotypes without direct human evidence.
- Kinetics of the accumulation of group 2 innate lymphoid cells in IL-33-induced and IL-25-induced murine models of asthma a potential role for the chemokine CXCL16 places ILC2 accumulation within IL-33/IL-25 airway models and links the process to CXCL16 as a candidate recruitment or positioning cue.
- Lipid mediator sources such as Lung type 2 innate lymphoid cells express cysteinyl leukotriene receptor 1 which regulates TH2 cytokine production and Cysteinyl leukotriene E(4) activates human group 2 innate lymphoid cells and enhances the effect of prostaglandin D(2) and epithelial cytokines support a lipid-amplified ILC2 activation branch.
- Human and therapeutic asthma comparators sharpen this branch: ICOS-ligand interaction is required for type 2 innate lymphoid cell function, homeostasis, and induction of airway hyperreactivity adds an ILC2 costimulatory and homeostatic amplifier of airway disease, The Role of the TL1A/DR3 Axis in the Activation of Group 2 Innate Lymphoid Cells in Subjects with Eosinophilic Asthma links airway eosinophilic asthma to a TL1A/DR3 activation axis, Fevipiprant, a selective prostaglandin D2 receptor 2 antagonist, inhibits human group 2 innate lymphoid cell aggregation and function defines a human DP2-blockade branch, and Lipid-Droplet Formation Drives Pathogenic Group 2 Innate Lymphoid Cells in Airway Inflammation shows that pathogenic airway ILC2 states are metabolically specialized rather than generic.
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Pulmonary environmental cues drive group 2 innate lymphoid cell dynamics in mice and humans adds a disease-positioning layer in which activated pulmonary ILC2s navigate peribronchial and perivascular niches through CCR8-CCL8 and collagen-I-dependent guidance.
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A population of c-kit+ IL-17A+ ILC2s in sputum from individuals with severe asthma supports ILC2 to ILC3 trans-differentiation adds human severe-asthma sputum evidence that intermediate ILC2s with c-kit and IL-17A features are enriched in mixed granulocytic airway inflammation and associate with neutrophilia.
- The molecular and epigenetic mechanisms of innate lymphoid cell (ILC) memory and its relevance for asthma strengthens the allergen-memory branch by linking mouse memory-like ILC2 recall responses to chromatin accessibility and transcriptional preparedness programs.
- Tissue-Restricted Adaptive Type 2 Immunity Is Orchestrated by Expression of the Costimulatory Molecule OX40L on Group 2 Innate Lymphoid Cells adds a mouse ILC2-OX40L branch in which IL-33-activated ILC2s license tissue-restricted Th2/Treg responses during pulmonary type 2 inflammation.
- ILC2s regulate adaptive Th2 cell functions via PD-L1 checkpoint control adds a mouse lung ILC2-PD-L1 branch in which activated ILC2s promote CD4 T-cell GATA3/IL-13 and primary helminth-associated type 2 immunity; this is a disease-relevant adaptive interface, not direct human asthma proof.
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Cross-talk between ILC2 and Gata3high Tregs locally constrains adaptive type 2 immunity adds a lung type 2 restraint branch in which ILC2-supported Gata3high Tregs limit effector-memory Th2 expansion and allergic inflammation through OX40L/OX40-linked feedback.
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IL-9 and Blimp-1 protect the transcriptional identity of group 2 innate lymphocytes in allergic asthma adds a mouse allergic-asthma ILC2 identity branch: IL-9-induced Blimp-1 supports IL-5/IL-13 type 2 output and limits type 1 cytokine deviation, while Blimp-1 loss also increases IL-9 and mast-cell recruitment.
- Severe asthma is characterized by a sex-specific ILC landscape and aberrant airway profile that is suppressed by anti-IL-5/5Ralpha biologics adds human severe-asthma blood/sputum evidence that airway ILC2 signatures, more than blood ILC2 signatures, align with reduced lung function; anti-IL-5/5Ralpha biologics selectively reduce IL-5+/IL-13+ airway ILCs without reducing core airway ILC2 abundance.
Respiratory viral infection and repair
- Innate lymphoid cells mediate influenza-induced airway hyper-reactivity independently of adaptive immunity supports a viral-triggered ILC axis that can drive airway hyperreactivity independently of adaptive immunity.
- Innate lymphoid cells promote lung-tissue homeostasis after infection with influenza virus supports the complementary idea that lung ILCs can promote tissue homeostasis and repair after influenza injury.
- Innate lymphoid cells in lung infection and immunity is useful as a review-level route across viral, bacterial, fungal, and helminth lung-infection contexts, but primary infection sources should anchor mediator-specific claims.
- Innate lymphoid cells integrate sensing and plasticity to control fungal infections provides primary mouse pulmonary fungal-infection evidence for ILC sensing, antifungal response modulation, and cytokine-driven ILC plasticity.
- BATF promotes group 2 innate lymphoid cell-mediated lung tissue protection during acute respiratory virus infection refines the repair branch by connecting BATF, wound-healing-enriched ILC2 states, and tissue protection during acute respiratory viral infection.
- Pulmonary IL-33 orchestrates innate immune cells to mediate respiratory syncytial virus-evoked airway hyperreactivity and eosinophilia supports an RSV-associated IL-33-ILC2-IL-13 airway hyperreactivity branch that should be interpreted separately from influenza repair biology.
- IL-1beta prevents ILC2 expansion, type 2 cytokine secretion, and mucus metaplasia in response to early-life rhinovirus infection in mice shows that early-life viral airway disease can include ILC2-dependent type 2 pathology but also cytokine brakes that restrain ILC2 expansion and mucus outcomes.
- Dampening type 2 properties of group 2 innate lymphoid cells by a gammaherpesvirus infection reprograms alveolar macrophages supports a non-canonical infection-conditioned ILC2 role: reduced type 2 output but increased GM-CSF-dependent imprinting of monocyte-derived alveolar macrophages.
- Innate type 2 lymphocytes trigger an inflammatory switch in alveolar macrophages adds a distinct lung niche-remodeling branch in which IL-33-activated ILC2-derived IL-13 reprograms tissue-resident alveolar macrophages toward an IRF4-driven inflammatory state.
Non-type-2 and plastic disease states
- IL-17-producing ST2(+) group 2 innate lymphoid cells play a pathogenic role in lung inflammation supports an IL-17-producing ST2+ ILC2-like pathogenic branch in lung inflammation.
- IL-1beta, IL-23, and TGF-beta drive plasticity of human ILC2s towards IL-17-producing ILCs in nasal inflammation supports the broader concept that inflammatory cytokine combinations can shift human ILC2s toward IL-17-producing programs, although nasal inflammation should not be treated as direct lung evidence.
- c-Kit-positive ILC2s exhibit an ILC3-like signature that may contribute to IL-17-mediated pathologies supports a c-Kit+ ILC2/ILC3-like interface that may matter for IL-17-linked pathology.
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Mechanics-activated fibroblasts promote pulmonary group 2 innate lymphoid cell plasticity propelling silicosis progression supports a silicosis branch in which mechanically activated fibroblasts promote ILC2 plasticity toward ILC1-like inflammatory output; this should not be merged into allergic asthma without the silicosis/fibrosis label.
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ILC2-derived LIF licences progress from tissue to systemic immunity adds a lung immune-egress branch in which ILC2-derived LIF controls pulmonary lymphatic CCL21 and CCR7+ immune-cell migration to lymph nodes, with different consequences in viral infection versus chronic allergen challenge.
- S1P-dependent interorgan trafficking of group 2 innate lymphoid cells supports host defense adds mouse evidence that inflammatory ILC2s can reach lung from intestinal tissue through S1P-dependent trafficking during type 2 challenge.
Niche-positioned and interferon-regulated disease roles
- Adventitial Stromal Cells Define Group 2 Innate Lymphoid Cell Tissue Niches reframes lung ILC2 disease activity as niche-positioned: ILC2s sit near ASCs that provide IL-33/TSLP and receive reciprocal IL-13-linked feedback.
- Pulmonary environmental cues drive group 2 innate lymphoid cell dynamics in mice and humans adds that activated pulmonary ILC2s are not only niche-positioned but dynamically guided by chemokine and extracellular-matrix cues.
- Innate type 2 lymphocytes trigger an inflammatory switch in alveolar macrophages shows that niche consequences can extend beyond cell positioning: activated ILC2s can directly reprogram tissue-resident alveolar macrophage state and thereby remodel the inflammatory alveolar compartment.
- Chitin activates parallel immune modules that direct distinct inflammatory responses via innate lymphoid type 2 and gamma delta T cells supports a model in which ILC2s drive eosinophil/AAM type 2 inflammation while restraining a parallel IL-17A/neutrophil module.
- IFN-gamma increases susceptibility to influenza A infection through suppression of group II innate lymphoid cells adds a viral-protection branch: IFN-gamma can suppress protective ILC2 IL-5/amphiregulin output during H1N1 infection without changing viral clearance in the reported mouse model.
- Interferon gamma constrains type 2 lymphocyte niche boundaries during mixed inflammation adds a mixed-inflammation branch in which IFN-gamma confines ILC2/Th2 cells to adventitial niches and limits pathogen-associated mortality.
Tumor and innate checkpoint context
- ILC2-driven innate immune checkpoint mechanism antagonizes NK cell antimetastatic function in the lung adds a non-asthma pulmonary disease branch where ILC2-associated checkpoint biology can suppress NK-cell antimetastatic function in the lung.
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Long-acting muscarinic antagonist regulates group 2 innate lymphoid cell-dependent airway eosinophilic inflammation and Cannabinoid receptor 2 engagement promotes group 2 innate lymphoid cell expansion and enhances airway hyperreactivity show that airway ILC2 disease can also be modulated by therapeutic cholinergic blockade or receptor-level cannabinoid amplification.
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Mesenchymal Stem Cells Suppress Severe Asthma by Directly Regulating Th2 Cells and Type 2 Innate Lymphoid Cells and Vitamin D3 resolved human and experimental asthma via B lymphocyte-induced maturation protein 1 in T cells and innate lymphoid cells add restraint branches for type 2 asthma, but neither should be treated as ILC2-exclusive clinical proof.
Therapy and Extrapulmonary Mechanism Context
- Immunotherapy for asthma supports endotype-aware therapy framing for asthma, but it should not be treated as primary evidence for a specific ILC2 mechanism.
- Gut ILC2 sources now add AHR, RXRgamma, ADM2, and tuft-cell IL-17RB/IL-25 regulatory branches. These are useful comparators for type 2 restraint, repair, and alarmin bioavailability, but direct pulmonary disease claims require lung, airway, sputum, BAL, or bronchial evidence.
Interpretation
The safest disease-level model is that ILC2s are lung tissue-response amplifiers whose role depends on the type of epithelial injury, inflammatory context, and timing. In allergic asthma, ILC2s are usually disease-amplifying through IL-5/IL-13, mucus, eosinophilia, and airway hyperresponsiveness. In respiratory viral infection, ILC2s are context-dependent: they can contribute to airway hyperreactivity, but they can also promote tissue repair and protective resolution programs.
The disease interpretation should separate three layers:
cell abundance: whether ILC2s expand or accumulate in lung, airway, sputum, blood, or tissue.effector output: whether ILC2s produce IL-5, IL-13, amphiregulin, IL-17, GM-CSF, or other mediators.disease outcome: whether the measured result is airway hyperresponsiveness, mucus metaplasia, eosinophilia, neutrophilia, lung injury, repair, or tumor control.
Treating these layers as interchangeable would overstate the evidence.
flowchart TD
accTitle: ILC2 Disease Outcomes
accDescr: Working map of ILC2 roles across lung disease states in the current wiki source set.
cues["Tissue cues"]
ilc2["Lung ILC2"]
type2["Type 2 output<br/>IL-5, IL-13"]
repair["Repair output<br/>AREG"]
plastic["Plastic output<br/>IL-17, GM-CSF"]
asthma["Allergic disease<br/>AHR, mucus"]
virus["Viral outcomes<br/>AHR or repair"]
niche["Niche effects<br/>AM or NK"]
cues --> ilc2
ilc2 --> type2 --> asthma
ilc2 --> repair --> virus
ilc2 --> plastic --> asthma
plastic --> niche
classDef cue fill:#e8f3ff,stroke:#3b6ea8,stroke-width:2px,color:#17324d
classDef cell fill:#fff4de,stroke:#b47a1f,stroke-width:2px,color:#4a3108
classDef output_class fill:#f6eefc,stroke:#7a55a3,stroke-width:2px,color:#2d1645
classDef disease fill:#eef7ed,stroke:#4d8a50,stroke-width:2px,color:#173d1d
class cues cue
class ilc2 cell
class type2,repair,plastic output_class
class asthma,virus,niche disease
Contradiction and supersession
- Contradiction: ILC2s can worsen airway inflammation in asthma models but support repair after viral injury. These are context-specific roles, not mutually exclusive claims.
- Contradiction: ILC2s are commonly type 2 cytokine producers, but multiple sources point to plastic IL-17-producing or ILC3-like disease states.
- Contradiction: viral infection can either trigger ILC-associated airway hyperreactivity or dampen type 2 ILC2 properties depending on viral model and timing.
- Supersession: no current source supersedes the full ILC2 disease model. The working strategy is to partition by disease, species, model, and timepoint.
Open questions
- Which ILC2 disease branch is most relevant to the user's current project: allergic asthma, respiratory virus infection, repair, or macrophage/niche reprogramming?
- In the project data, are ILC2s measured by flow phenotype, scRNA-seq cluster, cytokine protein, or inferred marker score?
- Are the project-relevant ILC2s canonical type 2 cells, memory-like cells, repair-like cells, or IL-17/ILC3-like plastic cells?
- Does the local dataset distinguish resident lung ILC2s from recruited or tissue-conditioned ILC2s?
- Which disease endpoint matters most: AHR, mucus, eosinophilia, neutrophilia, epithelial repair, macrophage state, or tissue damage?
Related pages
Future Expansion Directions
This short appendix highlights future literature directions rather than current disease conclusions. The most useful additions for later versions of this page would be:
- Primary asthma sources that cleanly separate mouse perturbation evidence from human association evidence.
- Respiratory-virus ILC2 studies resolved by timepoint, especially acute AHR, tissue repair, and post-infection macrophage or niche imprinting.
- Additional lung-compartment studies that sharpen human airway subsets, steroid-resistant asthma, and plastic IL-17 boundary states within ILC2.